https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30119 Wed 15 Dec 2021 16:11:02 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30416 Wed 11 Apr 2018 17:10:58 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26544 Wed 11 Apr 2018 15:40:57 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:28251 Wed 11 Apr 2018 14:22:59 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:4249 Wed 11 Apr 2018 13:10:54 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:18545 Wed 11 Apr 2018 12:52:16 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:16003 Wed 11 Apr 2018 12:17:35 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1900 Wed 11 Apr 2018 11:39:12 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:23271 Wed 11 Apr 2018 10:32:06 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24536 Wed 11 Apr 2018 09:53:13 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33337 Wed 06 Apr 2022 14:02:17 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:53966 4 and DNA damage repair), with the relevance of this to the UV sensitivity of folate and UV photosynthesis of vitamin D explained in detail, including the relevance of these vitamins to reproductive success. It explores whether we might be able to predict vitamin‐related gene polymorphisms that pivot metabolism to the prevailing UVR exposome within the vitamin D‐folate evolutionary hypothesis context. This is discussed in terms of a primary adaptive phenotype (pigmentation/depigmentation), a secondary adaptive phenotype (flexible metabolic phenotype based on vitamin‐related gene polymorphism profile), and a tertiary adaptive strategy (dietary anti‐oxidants to support the secondary adaptive phenotype). Finally, alternative evolutionary models for pigmentation are discussed, as are challenges to future research in this area.]]> Tue 23 Jan 2024 14:38:17 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39975 Tue 04 Oct 2022 15:09:23 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36011 Thu 17 Jun 2021 16:06:18 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43277 Thu 15 Sep 2022 12:09:32 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:12934 Sat 24 Mar 2018 10:36:37 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:28070 Sat 24 Mar 2018 10:21:27 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7520 Sat 24 Mar 2018 08:38:28 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:6984 Sat 24 Mar 2018 08:37:50 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:6997 T-MTHFR, 1298A>C-MTHFR, 80G>A-RFC, 2756A>G-MS, 66A>G- MSR, 19bpDHFR and 1561C>T-GCPII), only 677C>T-MTHFR was a significant risk for hypertension: OR 1.89; 95% CI 1.07–3.32 (χ² p = 0.038). Additionally, hypertensive subjects had a significantly lower intake of dietary folate than normotensive individuals (p = 0.0221), although this did not markedly alter blood metabolite levels. Several significant linear associations between dietary folate and related blood metabolites were found in normotensive subjects (p<0.001 for Hcy, red cell and serum folate) and were as predicted on an a priori basis – generally weaker associations existed in hypertensive subjects (p<0.05 for serum folate). This was true for data examined collectively or by genotype. Multiple regression analysis for diastolic or systolic blood pressure showed significant interaction for gender and folate intake (p = 0.014 and 0.019, respectively). In both cases this interaction occurred only in females, with higher folate intake associated with decreased blood pressure. Regressing diastolic blood pressure and 677C>T-MTHFR genotype showed significance (males; p = 0.032) and borderline significance (all subjects). Conclusion: Dietary folate and 677C>T-MTHFR genotype may modify blood pressure.]]> Sat 24 Mar 2018 08:37:49 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1162 Sat 24 Mar 2018 08:28:43 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10268 Sat 24 Mar 2018 08:13:06 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10795 Sat 24 Mar 2018 08:08:18 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:21473 Sat 24 Mar 2018 08:03:41 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19855 Sat 24 Mar 2018 07:57:05 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17921 Sat 24 Mar 2018 07:56:14 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20321 Sat 24 Mar 2018 07:55:10 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5558 Sat 24 Mar 2018 07:49:12 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27963 Sat 24 Mar 2018 07:38:45 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:28911 Sat 24 Mar 2018 07:26:00 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22266 Sat 24 Mar 2018 07:17:33 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:23349 Sat 24 Mar 2018 07:13:33 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37803 MTRR, MTR, MTHFR, CBS, SHMT1, MTHFD1, RFC1, BHMT, TYMS) and the Fitzpatrick skin phototype of populations was assessed via collation of genotypic data from ALFRED (Allele Frequency Database) and 1000 Genomes databases. Results: A significant association between variant frequency and Fitzpatrick phototype was observed for 16 of 17 examined variants (P<.0029 Bonferroni corrected significance threshold in all cases). Conclusions: These findings demonstrate novel relationships between skin color and folate-related genes, with trends suggesting folate genotypes are selected to maintain homeostasis in the folate system under differing UVR conditions.]]> Mon 26 Apr 2021 11:48:42 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37802 n = 619). Red blood cell folate, 25-hydroxyvitamin D (25(OH)D), and plasma Hcy levels were determined, and genotyping for 21 folate and vitamin D-related variants was performed. Erythemal dose rate accumulated over six-weeks (6W-EDR) and four-months (4M-EDR) prior to clinics were calculated as a measure of environmental UVR. Multivariate analyses found interactions between 6W-EDR and 25(OH)D levels (p_interaction = 0.002), and 4M-EDR and MTHFD1-rs2236225 (p_interaction = 0.006) in predicting Hcy levels. The association between 6W-EDR and Hcy levels was found only in subjects within lower 25(OH)D quartiles (<33.26 ng/mL), with the association between 4M-EDR and Hcy occurring only in subjects carrying the MTHFD1-rs2236225 variant. 4M-EDR, 6W-EDR, and MTHFD1-rs2236225 were also independent predictors of Hcy. Findings highlight nutrient-environment and gene-environment interactions that could influence the risk of Hcy-related outcomes.]]> Mon 26 Apr 2021 11:34:35 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37801 MC1R-rs1805007, IRF4-rs12203592 and HERC2- rs12913832) on folate (red blood cell (RBC) and serum) and homocysteine levels were examined in an elderly Australian cohort (n = 599). Genotypes were assessed by RT/RFLP-PCR, and UVR exposures were assessed as the accumulated erythemal dose rate accumulated over 4 months (4M- EDR). Multivariate analysis found significant negative associations between 4M-EDR and RBC folate (p, < 0.001, ß = -0.19), serum folate (p = 0.045, ß = -0.08) and homocysteine levels (p < 0.001, ß = -0.28). Significant associations between MC1R-rs1805007 and serum folate levels (p = 0.020), and IRF4-rs12203592 and homocysteine levels (p = 0.026) occurred but did not remain significant following corrections with confounders. No interactions between 4M-EDR and pigmentation variants in predicting folate/homocysteine levels were found. UVR levels and skin pigmentation-related variants are potential determinants of folate and homocysteine status, although, associations are mixed and complex, with further studies warranted.]]> Mon 26 Apr 2021 11:13:36 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29934 MTHFR-C677T, MTHFR-A1298C, cSHMT-C1420T TYMS 28bp 2R>3R, TYMS 3'UTR ins/del and DHFR 19bp deletion. Data extracted were analysed by the latitude of the study locations, as a surrogate measure of population UV exposure. Results: Frequency of the MTHFR-C677T and MTHFR-A1298C polymorphisms was positively associated with latitude, while a negative association was observed between latitude and frequency of the cSHMT-C1420T and TYMS 28bp 2R>3R variants. Conclusions: These findings provide novel evidence suggestive of folate genotypes being selected to maintain homeostasis between folate-dependent de novo thymidylate synthesis and methylation pathways in environments of differing UV levels. To the authors' knowledge, this is the first study to report significant associations between latitude and the occurrence of MTHFR-A1298C, TYMS 28bp 2R>3R and cSHMT-C1420T polymorphisms. On-going studies are required to further explore the biological significance of these findings.]]> Mon 26 Apr 2021 09:49:26 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32812 Mon 23 Sep 2019 10:19:59 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52534 Mon 16 Oct 2023 14:56:57 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37796 Fri 23 Apr 2021 12:32:04 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:42277  3R-TS. RCF was measured by chemiluminescent immunoassay and vitamin D2 and D3 by HPLC. Results: RCF and photosynthesized vitamin D3, but not RCF and dietary vitamin D2, exhibit a significant reciprocal association in spring and summer. Three folate genes (C677T-MTHFR, C1420T-SHMT, and 2R > 3R-TS) strengthen this effect in spring, and another (T401C-MTHFD) in summer. Effects are seasonal, and do not occur over the whole year. Conclusions: Findings are consistent with what might be required for the “folate-vitamin D-UV hypothesis of skin pigmentation” model. It suggests genetic influence in provision of one-carbon units by 5,10-methylene-H4folate, may be an important factor in what appears to be a clear seasonal relationship between vitamin D3 and folate status.]]> Fri 11 Aug 2023 09:43:44 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37039 Fri 07 Aug 2020 12:28:15 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24987 in vitro model. Methods: Caco-2 (colorectal cancer) and MCF7 (breast cancer) cell lines were cultured at 6 different PteGlu concentrations (0, 0.1, 1, 50, 250, and 500µg/ml) for 6 days. Cell growth was determined using thiazolyl blue tetrazolium bromide assay. The genotype of dihydrofolate reductase 19bp deletion/insertion (DHFR 19-del) was also scored in cell lines using a restriction fragment length polymorphism technique to examine whether genetic variations may factor in cell proliferation. Results: PteGlu exhibited differential growth promoting properties between cell lines. Caco-2 cells did not show a significant growth difference at low concentrations compared to control, however, at higher concentrations, the growth showed a contrasting trend in the early experimental period, while MCF7 showed enhanced cell growth at all concentrations. The DHFR 19-del genotype differed in the two cell lines. Conclusions: Altered response to PteGlu by Caco-2 and MCF7 may reflect a tissue specific disease aetiology or genotype specific differential enzyme activity, for example by DHFR, to critical levels of PteGlu. As folic acid fortification is a blanket intervention, and DHFR and other enzyme activities vary between individuals, PteGlu intake may have an as yet undefined effect on health. These findings may be relevant when considering mandatory folic acid fortification for disease prevention.]]> Fri 03 Dec 2021 10:34:46 AEDT ]]>